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. 2022 Jan 31;58:101446. doi: 10.1016/j.molmet.2022.101446

Figure 1.

Figure 1

Survivin expression in adipose tissue and adipocytes is regulated by nutritional stress both in vivo and in vitro. (A) Survivin expression in iWAT of 8-week-old mice under fasting (16 h) or refeeding (4 h) conditions was determined by western blot. (B–D) Birc5 gene expression in the iWAT (B), eWAT (C), and BAT (D) of 14-week-old mice fed with normal CD or HFD for 3 days or 1 week, as determined by qRT-PCR. (E) Survivin expression was detected by western blot in 3T3-L1 adipocytes, which were treated with branched chain amino acid (BCAA) for the indicated times. (F) Survivin expression in 3T3-L1 adipocytes treated with phosphatidic acid (PA) at the indicated concentrations for 12 h. (G, H) Survivin expression in 3T3-L1 adipocytes treated with 3-benzyl-5-((2-nitrophenoxy) methyl)–dihydrofuran-2 (3 h)-one (3BDO, an inhibitor of mTORC1) at the indicated concentrations for 24 h (G) or with 30 μM 3BDO for the indicated times (H). (I) Survivin expression in differentiated beige adipocytes treated with 3BDO at the indicated concentrations for 24 h. (J) Protein levels of survivin in 3T3-L1 adipocytes overexpressing survivin after withdrawing FBS or/and glucose for 3 h, as detected by western blot. (K) Survivin was detected in 3T3-L1 adipocytes overexpressing survivin under FBS-free conditions in the presence of 100 nM insulin, 10 μM MG132, and 60 μM CQ. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.