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. 2022 Jan 31;58:101446. doi: 10.1016/j.molmet.2022.101446

Figure 5.

Figure 5

Survivin was essential for the thermogenic program and mitochondrial respiration of thermogenic adipocytes in vitro. (A) Glycerol release adjusted to cellular protein content under basal conditions or after treatment with 5 μmol/L isoproterenol (ISO) for 3 h in differentiated brown adipocytes from Con and SKO mice (n = 3). (B) Expression of thermogenic genes was detected by qRT-PCR in differentiated brown adipocytes from Con and SKO mice (n = 3). (C) Expression of survivin and the thermogenic protein Ucp1 in differentiated brown adipocytes from Con and SKO mice was determined by western blot. (D) Oxygen consumption rate (OCR) measurement of differentiated brown adipocytes from Con and SKO mice (n = 5). (E) Glycerol release adjusted to cellular protein content under basal conditions or after treatment with 5 μmol/L ISO for 3 h in differentiated beige adipocytes from Con and SKO mice (n = 4). (F) Expression of thermogenic genes in differentiated beige adipocytes from Con and SKO mice. (G) Expression of survivin, Pgc1α, and Ucp1 in differentiated beige adipocytes from Con and SKO mice. (H) OCR measurement of differentiated beige adipocytes from Con and SKO mice (n = 5). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.