Figure 6.

Adipocyte-specific deletion of survivin induced autophagy in vivo and in vitro. (A) Protein expression of SQSTM1/p62 in BAT of 24-week-old Con and SKO mice fed CD. (B) Protein expression of SQSTM1/p62 in BAT of 9-week-old Con and SKO mice after 3 days of cold exposure. (C) Representative TEM imaging of BAT of Con and SKO mice after 3 days of cold exposure. (D) Immunoblotting for SQSTM1/p62 and Ucp1 of differentiated beige adipocytes from Survivin^flox/flox mice infected with Conv or Ad-Cre and treated with 0.2% BSA for 4 h. (E) The iWAT SVFs were extracted from Survivin^flox/flox mice, differentiated into beige adipocytes, and successively infected with Conv or Ad-Cre and Ad-mRFP-GFP-LC3. Images were acquired using a confocal laser scanning microscope after culturing cells in fresh complete DMEM containing 10% FBS or serum starvation medium supplemented 0.2% BSA for 2 h.