Figure 2.

Immunofluorescence analysis of Nrf2 nuclear translocation (n=6 in each group). (A) Kupffer cells were isolated from the WT mice that underwent the different experimental procedures and were fixed immediately. Nrf2 was probed with a primary anti-Nrf2 antibody and visualized with a goat anti-Rabbit DyLightH 488-conjugated secondary antibody. The nuclei were stained with DAPI. The images were taken using confocal fluorescence microscopy. (B) The quantitative analyses of the mean intensity of fluorescence (λ = 488nm) in the nuclei were performed with the AxioVision Rel. 4.8 software (Carl Zeiss Micro Imaging, LLC) (*p < 0.05, **p < 0.01; One-way ANOVA followed by Tukey’s post hoc test).