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. 2022 Feb 10;13:813218. doi: 10.3389/fimmu.2022.813218

Figure 4.

Figure 4

The effect of CD39/CD73 inhibiting on the cytokine secretion of dNK cells. (A) dNK cells were isolated from decidual tissues of normal pregnant women (n=4) and URSA patients (n=4), and cultured in a 24-well plate (2 × 105 cells/well) for 24 hours. The cytokine concentrations in the media were determined using Luminex assay (mean ± SEM, ns, statistically not significant, *P < 0.05, ***P < 0.001, two-tailed t tests). (B) Cytokine concentrations in the media of dNK cells (n=5) cultured in the absence or presence of the CD39 inhibitor ARL67156 in 24-well plates for 24 hours (mean ± SEM, ns, statistically not significant, *P < 0.05, **P < 0.01, two-tailed t tests). (C) Cytokine concentrations in the media at 24 hours of co-culture of dNK and HTR-8/SVneo cells (n=3) (mean ± SEM, ns, statistically not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with the untreated group, one-way ANOVA and post hoc tests).