Skip to main content
. 2022 Feb 10;9:790995. doi: 10.3389/fmed.2022.790995

Figure 2.

Figure 2

Effects of TGF-β on expression and distribution of VISTA in various human cell types. (A) Jurkat T and TALL-104 cells were exposed to 2 ng/ml TGF-β for 24 h followed by total RNA isolation and detection of VISTA mRNA using qRT-PCR as outlined in Materials and Methods; (B) Resting Jurkat T cells and those treated for 24 h with 2 ng/ml TGF-β were subjected to ChIP followed by qRT-PCR as described in Methodology section in order to detect whether Smad3 binds to the VSIG promoter region; (C) Resting as well as PMA-activated Jurkat T cells, TALL-104 and THP-1 cells were exposed for 24 h to 2 ng/ml TGF-β followed by detection of cell surface-based VISTA by on-cell Western (see Materials and Methods for details); Jurkat T cells (D), primary human T lymphocytes (E), THP-1 AML cells (F), primary AML blasts (G) and HaCaT keratinocytes (H) were exposed to 2 ng/ml TGF-β for 24 h followed by detection of cell-associated (gray color) and secreted (black color) VISTA levels. Images are from one experiment representative of 5 which gave similar results. Data are the mean values of 5 independent experiments. * p < 0.05 vs. control (total VISTA).