Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 23;13(2):174. doi: 10.1038/s41419-022-04624-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — A Volcano plot of all expressed genes is shown to identify DEGs obtained from RNA-seq data in SAPCD2-knockdown SK-N-BE(2) cells with abs(log₂(fold change)) >1 and adjusted p value <0.05. B, C KEGG pathway analysis (B) and GO analysis (C) for DEGs. D Enrichment plots generated from GSEA hallmark gene set using DEGs upon SAPCD2-knockdown. NES normalized enrichment score. NOM p value, adjusted p value. E Activity of the dual-luciferase reporter vector containing WT or mutant E2F-binding motifs that were transfected into SK-N-BE(2) cells transfected with sh-Scb or sh-SAPCD2. F qPCR analysis of mRNA levels for the indicated E2F transcription activators, E2F target genes, and cell cycle-related genes in SK-N-BE(2) cells transfected with sh-Scb or sh-SAPCD2. Unpaired two-sided t-test for analysis in E–F, data were shown as mean ± SD (error bars). ns, not significant. Data were representative of three independent experiments.