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. 2022 Feb 23;13(2):174. doi: 10.1038/s41419-022-04624-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Motif enrichment analysis by cisTarget database revealing the potential upstream transcription factors of the downregulated genes in the SAPCD2-knockdown SK-N-BE(2) cells. NES, normalized enrichment score of the motif in the gene set. AUC, area under the curve. TF_highConf, transcription factors annotated to the motif according to the “motifAnnot_highConfCat” dataset. nEnrGenes, Number of genes highly ranked. rankAtMax, Ranking at the maximum enrichment. B Immunoprecipitation of endogenous SAPCD2 or E2F7 in SK-N-BE(2) cells analysed by western blot showing an interaction between SAPCD2 and E2F7. Rabbit IgG was used as a negative control. C Immunofluorescence confocal images showing the interaction between SAPCD2 and E2F7 in SK-N-BE(2) cells stably transfected with FLAG-tagged SAPCD2. Arrow, SAPCD2 appears to be mainly colocalized with E2F7 in the cytoplasm. Scale bars, 10 μm. D, E Immunofluorescence confocal images and western blot showing the subcellular distribution of E2F7 in SK-N-BE(2) cells transfected with sh-Scb or sh-SAPCD2 for 72 h. Scale bars, 10 μm. F, G Activity of E2F luciferase reporter that were co-transfected into 293T cells with increasing amounts of E2F7 expression plasmid (100–500 ng) in the presence or absence of constant amounts (500 ng) of E2F7 expression plasmid. H Heatmap revealing the expression of predicted E2F7-targeted genes obtained from RNA-seq data in SAPCD2-knockdown SK-N-BE(2) cells and public dataset of NB patients with different survival time (GSE16476). I, J Scatter plot showing a positive significant correlation in transcript level between SAPCD2 and HMGA1 or BRCA1 in NB tissues. Unpaired two-sided t-test for analysis in F, G, data were shown as mean ± SD (error bars). ns, not significant. Pearson’s correlation coefficient in I, J. Data were representative of three independent experiments.