Fig. 2. NETs promote GC cells invasion and migration in vitro and metastasis in vivo.

A, B MKN-45 (A) and MGC-803 (B) cells as indicated neutrophils and treatment were subjected to transwell Matrigel invasion and migration assays; C, D Representative images (left) and quantification (right) of metastatic lesions in luciferase-MKN-45 cells bearing LM (C) and PM (D) nude mice models. After coculturing with neutrophils from indicated groups and treatment, luciferase-MKN-45 cells were injected to spleen (LM model) or abdominal cavity (PM model) for metastasis. Data represent the mean ± S.D. in A, B (n = 3 biologically independent experiments) and C, D (n = 5 per group); one-way ANOVA with Tukey test was used in A, B, C, D. Source data are provided as a Source Data file.