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. 2022 Feb 23;13:1017. doi: 10.1038/s41467-022-28492-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A, B Left panel: representative immunofluorescence co-staining of DNA, E-cadherin and N-cadherin in MKN-45 (A) and MGC-803 (B) cells as indicated treatments (PADi, NEi, or DNase I were used to inhibit NETs specifically). Right panel: The mRNA levels of E-cadherin and N-cadherin in MKN-45 and MGC-803 cells as indicated treatments were assayed by quantitative reverse transcriptase (qRT)–PCR; C Representative HE (metastatic lesions) and immunofluorescence co-staining images of DNA, E-cadherin and N-cadherin in liver (upper panel), and peritoneum (down panel) collected from LM and PM nude mice (n = 5 per group) as indicated in Fig. 2C, D; D Representative HE, immunofluorescence co-staining images of DNA, Cit-H3, and MPO, immunohistochemical images for E-cadherin and N-cadherin in metastatic leasions from GC patients with LM (n = 3) or PM (n = 3); E Plasma and serum levels of MPO–DNA in GC patients as indicated in Fig. 3D. Data represent the mean ± S.D. in A, B (n = 3 biologically independent experiments) and D, E (n = 3 per group); one-way ANOVA with Tukey test was used in A, B; Unpaired Student’s t-tests were used in E. Source data are provided as a Source Data file.