Fig. 2.

Knockdown of FPR2 expression and inhibition of its activity affects the release of inflammatory cytokines in HTR8/SVneo cells. (A) Determination of FPR2 protein levels in HTR8/SVneo cells transfected with siFPR2 (***P < 0.001: siFPR2 vs siNC group). (B) Estimation of the levels of proinflammatory cytokines (TNF-α, IL-1β, IL-6) and of the anti-inflammatory cytokine IL-10 in HTR8/SVneo cells. The LPS concentration used was 100 ng/ml, whereas the siFPR2 concentration used was 600 ng/ml and the WRW4 concentration was 10 μg/ml (**P < 0.01: LPS vs CON group; + P < 0.05, +  + P < 0.01: LPS + siFPR2 or LPS + WRW4 vs LPS group)