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. 2022 Feb 2;58:101451. doi: 10.1016/j.molmet.2022.101451

Figure 1.

Figure 1

Tm4sf5−/−KO mice showed lower hepatic acylglycerols levels, compared to WT mice. Six-week-old C57BL/6 WT and Tm4sf5−/− KO male mice (n = 10) were fed adjusted-calorie diet (ACD) with a high sucrose content diet (325 g/kg, HSuD) for either 3 or 10 weeks. (A) Randomly selected mouse-liver sections underwent H&E staining. (B) Each animal's BW was measured every week and graphed for 10-week periods, whose area under the curves (AUC) were calculated and graphed. In addition, amounts of food intake by WT and KO mice were measured and compared at mean ± standard deviation values (g/animal/day) for the diet period. (C) GTT and ITT were performed using an intraperitoneal injection of either glucose or insulin in mice fed HSuD for 10 weeks. AUC values were also calculated and graphed. (D to F) Randomly selected WT mouse livers (n = 6) and KO mouse livers (n = 5) from 10-week ACD mice were processed for LC-MS/MS-based lipidomic analyses. Among the lipids analyzed, MAG, DAG, and TAG levels were graphed. ∗, ∗∗, ∗∗∗, and ∗∗∗∗ depict p values less than 0.05, 0.01, 0.005, or 0.001to represent statistical significance using student t test (B and C) or a two-way ANOVA (D to F).