Figure 4.

NEK7 regulated HCC pyroptosis and inhibited its migratory toward HSC. (A) Protein levels of NLRP3, GSDMD, NEK7, and Cleaved caspase-1 in HCCs transfected with shRNAs targeting NEK7 or negative control. LPS and nigericin treatment was positive control for cell pyroptosis. (B) IL-1β release of HCC supernatant. (C) LDH release of HCC supernatant. (D) shControl or shNEK7 cells were dyed with CellTracker Green, LX-2 cells were dyed with CellTracker Red, and migration was performed for 12 and 24 h. Original magnification: ×100. Scale bars = 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001.