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. 2022 Feb 10;12:764843. doi: 10.3389/fpls.2021.764843

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Copyright © 2022 Jhu, Farhi, Wang, Zumstein and Sinha.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Laser-capture microdissection (LCM) of C. campestris haustoria penetrating tomato stems at three developmental stages. (A–C) Toluidine blue O stained paraffin sections of tomato stem with C. campestris early (A), intermediate (B), and mature stage (C) haustoria. Red line indicates the interface between C. campestris and host tomato. Cc indicates C. campestris; Sl indicates S. lycopersicum. (D–F) C. campestris haustorial tissues and host tissues were collected using LCM. A paraffin section of an intermediate stage haustorium before collection (D), after haustorial tissue collection (E), and after host tissue collection (F). (A) and (C), scale bars = 250 μm. (B), (D), (E), and (F), scale bars = 100 μm. Part of this figure (A–C) is modified from one in a previously published paper Jhu et al., 2021 with new information added.