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. 2022 Feb 10;13:847087. doi: 10.3389/fpls.2022.847087

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Copyright © 2022 Hu, Song, Xu, Wei, Tao, Ren, Yu, Li, Hu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional analysis of TaSHMT3A-1 by Barley stripe mosaic virus-based virus-induced gene silencing (BSMV-VIGS) in Bainong207. (A) BSMV: TaSHMT3A-1 infected individual plants were inoculated with F. graminearum, and their leaves were photographed at 3 and 5 days post-inoculation (dpi). BSMV:γ were performed as a control. The experiment was repeated independently three times and the same results were obtained. Scale bar, 5 mm. (B) Expression of TaSHMT3A-1 at 14 days in BSMV: TaSHMT3A-1-infected leaves was compared with that in BSMV:γ-infected controls of Bainong207. CK represents plants inoculated with BSMV:γ, and 1-3 represents plants inoculated with BSMV: TaSHMT3A-1. Asterisks show significant differences compared with the control (Duncan’s honestly significant difference test), **P < 0.01.