Fig. 2.

The images show the single neuron trapping mechanisms on various microfluidic platforms. A The brain-on-a-chip concept schematic depicting a modified microsieve electrode array (μSEA) with pairing of single neurons to a single electrode, beneath is the 3-D brain tissue construct cultured atop the μSEA within a bioreactor [14]. B Analysis set-up (a) and working principles of the 3D microsieve array (b). (c) A Colour map can showing the presence of cells on the distinct microsieve pore via impedance characteristics [30]. C Images recorded for single neuron trapping on ring shaped trapping device arrays. (a) Incoming neuron (I) enters the 1st trap. (II) The neuron gets immobilized in the 1st trap electrode overcoming fluid flow. (III) After the neuron gets trapped, a repelled particle keeps moving forward with the flowing media. (IV) The 2nd trap captures the repelled neuron. (V and VI) Further neurons get trapped inside the 3rd and the 4th ring trap consecutively [31].