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. 2022 Feb 17;8(2):e08989. doi: 10.1016/j.heliyon.2022.e08989

Figure 2.

Figure 2

I3C prevented LPS-induced oxidative stress in mouse Bv-2 cells. (A) I3C reduced superoxide anion damage measured by dihydroethidium (DHE) fluorescence. (B) The quantitation confirmed the antioxidant protection by I3C. (C) NADPH activity was enhanced by LPS and effectively attenuated in the mitochondrial fractions (D) by I3C Oxidative stress markers induced by LPS. (E) The p22phox subunit of NADPH augments its levels in the presence of LPS, but this is neutralized in the presence of I3C. (F) LPS increased inducible nitric oxide synthase (iNOS) enzyme expression regardless of LPS stimulus and (G) NO levels increased in the stressed cells. I3C did not reduced NO levels. ∗p < 0.05 vs control and #p < 0.05, ##p < 0.01, ###p < 0.001 vs LPS.