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. 2022 Feb 24;32(4):375–382. doi: 10.1038/s41422-022-00630-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Design and encapsulation of an mRNA antibody mRNA-HB27-LNP. b HB27 antibody expression in Vero, 293 T and Expi293F cells at 24 h after transfection with mRNA-HB27 using ELISA assay. Data are shown as mean ± SEM. Data are analyzed by One-way ANOVA with multiple comparisons (****P < 0.0001). c Inhibition of SARS-CoV-2 infection in Vero cells at 24 h after transfection with 1 μg of mRNA-HB27 by Immunofluorescence assay. SARS-CoV-2 S protein was stained in green, and DAPI in blue. Scale bars, 100 μm. d, e The antibody concentration and NT₅₀ titer of serum samples in mice. Briefly, female BALB/c mice (n = 4/group) were i.v. administrated with the indicated dose of mRNA-HB27-LNP or Placebo. Then, mice sera at 24 h post administration were measured by ELISA (d) and SARS-CoV-2 pseudovirus neutralization assay (e), respectively. Dotted lines indicate the limits of detection. Data are shown as mean ± SEM. f Intravenous injection of reporter mRNA-LNPs for in vivo imaging in mice. IVIS Spectrum image (6 h post-injection) of female BALB/c mice were injected with 10 μg of FLuc-encoding reporter mRNA-LNP by the intravenous route.