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. 2022 Feb 10;15:835448. doi: 10.3389/fnmol.2022.835448

FIGURE 2.

FIGURE 2

Tmie mutant hair cells are more resistant to cisplatin ototoxicity. (A) Cochlear explants were dissected from P3 Tmie± and Tmie–/– mice and then exposed to cisplatin at different concentrations for 2 days. The samples were fixed and labeled with an antibody against β2-spectrin. The majority of the Tmie–/– hair cells were dead with a few swollen hair cells in the explants, after the exposure to 50 μM of cisplatin for 2 days. Note, the Tmie–/– hair cells are more resistant to cisplatin ototoxicity. Scale bar: 10 μm. (B) Numbers of hair cells per 100 μm in the middle region of the cochlea (5 mice per group, control; 3 mice per group, 5 μM cisplatin; 7 mice per group, 10 μM cisplatin; 4 mice per group, 20 μM cisplatin; 4 mice per group, 30 μM cisplatin; 3 mice per group, 50 μM cisplatin). Data are represented as the mean ± SE. n.s., not significant; ***p < 0.001 by Student’s t-test.