FIGURE 4.

Cimetidine protects wild-type hair cells from cisplatin ototoxicity. (A) Cochlear explants were dissected from P3 wild-type mice and then exposed to different concentrations of cisplatin with/without 1 mM cimetidine for 2 days. The samples were then fixed and labeled with an antibody against β2-spectrin. Note, 1 mM cimetidine partially protected hair cells from cisplatin ototoxicity. Scale bar: 10 μm. (B) Numbers of hair cells per 100 μm in the middle region of the cochlea (5 mice per group, 10 μM cisplatin; 6 mice per group, 20 μM cisplatin; 3 mice per group, 30 μM cisplatin; 3 mice per group, 50 μM cisplatin). Data are represented as the mean ± SE. ^**p < 0.01 and ^***p < 0.001 by Student’s t-test.