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. 2022 Feb 10;12:804113. doi: 10.3389/fneur.2021.804113

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Copyright © 2022 Bahauddin, Ivannikov, Wang, Jamaluddin, Curtis, Ibtehaj, Yeager, Soong, Fang and Huda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HDAC expression and HDAC vs. HAT activities in PBMCs. CFA and EAMG mice (± HDAC-i) were bled 72 h post 2nd and 3rd treatments. (A) Relative mRNA fold changes of HDAC isoforms 1, 2, and 3 in AChR-stimulated PBMCs from post 3rd treatment. (B) Relative HDAC/HAT total enzymatic activities in the whole-cell extract of AChR-stimulated PBMCs from post 2nd treatment. Data (fluorescence intensity) shown are the mean values of the pooled samples in wells assayed in duplicate (n = 3 for CFA; n = 5 for CA-PBS and each treatment groups). (C,D) Immunoblots showing H3K4ac and H4K20ac in whole-cell lysates of splenocytes from EAMG mice treated or not with HDAC-i. Bottom panel: nonspecific bands to show loading uniformity in the gel stained with Coomassie blue. The experiment was repeated thrice. ***P < 0.001, ANOVA.