Figure 5.

Frequency of (intracellular) IL-6-positive immune cell types. (A,B) CFA (n = 3) or EAMG mice (n = 5 per group) treated with HDAC-i were bled 72 h and 4 weeks post 3rd treatment for flow cytometric analyses of IL-6-producing cells. Isolated PBMCs and splenocytes were stimulated with AChR in presence of brefeldin for 26 h, fixed/permeabilized, and stained with specific fluorochrome-conjugated antibodies to enumerate IL-6-positive immune cell types. (C) An aliquot of splenocytes from EAMG mice (isolated 4 weeks post 3rd treatment) treated with HDAC-i and not stimulated but Fc-blocked and stained with BCMA-specific monoclonal Ab (Ray Biotech) and PE-conjugated secondary Ab. The numbers below the figures summarize the percent frequency changes of immune cells. Shown on the right are the percent frequencies of BCMA-expressing cells. The experiment is representative of three similar experiments. Three replicates of pooled samples per group were used for flow analyses.