Figure 6.

AChR-specific IgG2b levels in EAMG mice ± HDAC-i. (A,B) CFA (untreated) and EAMG mice were treated with HDAC-i and their blood was collected to obtain the serum at 72 h and 4 weeks post 3rd treatment. Serum dilutions were titrated to perform the ELISA for AChR-specific IgG2b (n = 3 for the CFA group, n = 5 for each CA or EAMG group). Each plot shows multiple plate readouts at a minute or less interval using 405-nm OD. (C) Crude preparation of muscle receptor from forelimb muscle of CFA and EAMG mice were analyzed for relative density of functional AChR via ELISA (using absorbance at 405 nm). **P < 0.01, ***P < 0.001, One-way ANOVA. n = 3 for the CFA group, n = 5 for each CA-PBS, DMSO, and HDAC-i treated EAMG group. The experiment is representative of three similar experiments.