TABLE 3.
Indirect and direct in vitro ovarian cancer co-culture models.
| Co-culture type | Co-culture model | Model format | References |
|---|---|---|---|
| Indirect/non-contact | Cancer-stroma | SK-OV-3 + FP-96 fibroblasts (transwell insert) | Medeiros et al. (2019) a |
| OVCAR-5 + MRC-5 fibroblasts (bioprinted onto Matrigel) | Xu et al. (2011) | ||
| Cancer-immune (macrophage) | SK-OV-3, HEY, HO8910, A2780 in Matrigel (transwell insert) + Primary macrophages | Yi et al. (2020) | |
| SK-OV-3 spheres (transwell insert) + THP-1 macrophages | Ning et al. (2018) | ||
| SK-OV-3 + THP-1 macrophages (transwell insert) | Wang et al. (2013) a | ||
| Cancer-endothelial | SK-OV-3, OVCAR-3 + HUVECs on Matrigel (transwell insert) | Yuan et al. (2021) a | |
| “NICO-1” transwell system—Primary OvCa stem cell spheroids (ascites, ULA plate) + HUEhT-1 endothelial cells on Matrigel | Miyagawa et al. (2020) | ||
| Cancer-MSC | OvCa cell lines + MSC (adipocyte, bone marrow, umbilical cord) conditioned media | Khalil et al. (2019) a | |
| Direct/contact | Cancer-stroma | SK-OV-3 on top of WI38 fibroblasts in Matrigel | Lau et al. (2017) |
| HEY or SK-OV-3 + NIH3T3 cells in hanging drop | Weydert et al. (2020) | ||
| A2780 + Human ovarian fibroblast cell line in a microfluidic chip | Flont et al. (2020) | ||
| SK-OV-3 + mesenchymal cells (MUC-9) or fibroblasts (CCD27-Sk) in ULA plates | Tofani et al. (2021) | ||
| Cancer-immune (macrophage) | ID8 cells on top of Matrigel + TAMs from mouse ascites | Long et al. (2018) | |
| OVCAR-3 + PBMCs in hanging drop | Raghavan et al. (2019) | ||
| Cancer-adipocyte | ID8 cells on top of primary mouse adipocytes in Matrigel | John et al. (2019) | |
| Cancer-mesothelial | OVCA433 spheroids (created on poly-HEMA coating) on top of immortalized human lung mesothelial cells or MeT-5A mesothelial cells | Iwanicki et al. (2011) | |
| CAOV-3 or A2780 + Primary mesothelial cell or MeT-5A mesothelial cell spheroids on poly-HEMA coated plates | Shishido et al. (2018) | ||
| OV-MZ-6 + MeT-5A mesothelial cells in PEG hydrogel | Loessner et al. (2019) | ||
| Organotypic omental mesothelial model | OvCa cell line + Primary mesothelial cells + Primary omental fibroblasts | Kenny et al. (2009); Kenny et al. (2007); Mitra et al. (2015a); Henry et al. (2017); Lu et al. (2019); Li et al. (2017); Peters et al. (2015) | |
| Multicellular models | OVCAR-4 + HUVEC + hMSC in peptide-based hydrogels | Hedegaard et al. (2020) | |
| Patient explant orbital rotational cultures (epithelial cells, fibroblasts, tumor-infiltrating immune cells) | Abreu et al. (2020) | ||
| Early passage HGSOC organoids (maintained immune cells) | Hill et al. (2018) |
a
Cancer cells grown in 2D
MSC, Mesenchymal stem cell; HUVEC, Human umbilical vein endothelial cell; CSC, Cancer Stem Cell; OvCa, Ovarian Cancer; TAM, tumor-associated macrophages; PBMC, primary blood mononuclear cell; PEG, polyethlyene glycol.