FIGURE 3.

Fire blight inoculation induces changes in transcriptome-wide m⁶A modification, resulting in the increase of RNA abundance of m⁶A modified transcripts. (A) Overlap of m⁶A-modified genes among three replicates of mock (blue circles) and 12 HPI (red circles), the intersection of replicates indicates high-confidence m⁶A-modified genes. The subset of 0 HAI and 12 HAI indicates specifically m⁶A-modified genes before and after blight inoculation. (B) Relative abundance of m⁶A-modified gene transcripts in mock to 12 HPI contain mock-specific (blue box), 12 HPI–specific (red box), and mock-12 HPI overlap (green box). *p < 0.05; **p < 0.01. (C) Cumulative distribution function (CDF) of the relative abundance (12 HPI divided by mock) of transcripts in mock-specific m⁶A-modified genes and 12 HPI–specific m⁶A-modified genes compared using the Wilcoxon t-test. (D–F) Expression pattern of m⁶A-modified genes at different time points after inoculation.