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. 2022 Feb 10;12:836517. doi: 10.3389/fonc.2022.836517

Figure 2.

Figure 2

The pathological characteristics, tumor occurrence, and the insertion sites of HBx fragment in Sleeping Beauty (SB) mouse models. (A) Representative images of H&E and IHC staining. The first column shows representative gross features indicating the tumors in the livers. The second column shows representative H&E stainings indicating the histological features of the livers. The third to fifth columns show representative IHC stainings for HBx, CK-18, and AFP. Vector, the mice injected with the empty vector; WT, M1, M2, M3, and Ct, the mice injected with vectors carrying WT-HBx, M1-HBx, M2-HBx, M3-HBx, and Ct-HBx, respectively. (B) Incidence of tumors in the mouse models. V, the mouse models injected with the empty vector, n=7; WT, the mouse models injected with WT-HBx, n=7; M1, the mouse models injected with M1-HBx, n=7; M2, the mouse models injected with M2-HBx, n=4; M3, the mouse models injected with M3-HBx, n=8; Ct, the mouse models injected with Ct-HBx, n=7. (C) The number of HBx insertion sites in different functional regions and different tissue types. T, tumors from the mice with tumor nodules; N, liver tissues from the tumor-free mice. (D) The total number of HBx insertion sites between two of the three groups. **P < 0.01.