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. 2022 Feb 10;13:816107. doi: 10.3389/fendo.2022.816107

Figure 4.

Figure 4

Function of BAT was impaired in adult offspring of LH and HH, but LH seemingly more badly. (A) UCP1 staining for BAT sections of 10-week-old LL, HH and LH mice fed LFD or HFD (n = 3, from 3 litters) and UCP1 positive area. mRNA expressions of thermogenic genes in BAT when fed LFD (B) or HFD (C) (n =3, from 3 litters). Western blot analysis for thermogenic marker (UCP1) in BAT of adult LL, HH, and LH mice fed LFD (D) or HFD (E) (n =3, from 3 litters). Western blot analysis for UCP1, p-HSL and HSL in iWAT of adult LL, HH, and LH mice fed LFD (F) or HFD (G) (n = 3, from 3 litters). The fold increase was determined and normalized to β-ACTIN. Data are expressed as the mean ± SEM. *p <0.05, **p <0.01: HH versus LL group. ^ p <0.05, ^^ p <0.01: LH versus LL group. # p <0.05, ## p <0.01: LH versus HH group. Scale bar, 100 μm (A).