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. 2022 Jan 20;298(3):101611. doi: 10.1016/j.jbc.2022.101611

Figure 2.

Figure 2

TRIM34 promotes the interaction between ZBP1 and RIP3.A, 293T cells were transfected with indicated ubiquitin plasmids. Forty-eight hours post-transfection, Co-IP and immunoblot analysis were performed with indicated antibodies. B, THP-1 macrophages were transfected with vector control or Flag-TRIM34 for 24 h. Then, cells were infected with IAV (MOI = 1) for 24 h or left uninfected. Co-IP and immunoblot analysis were performed with the indicated antibodies. C, THP-1 macrophages were transfected with siRNA-control (si-ctrl) or siRNA-TRIM34 (right panel) for 48 h prior to qPCR (upper panel) and Western blot assays (lower panel). D, THP-1 macrophages were transfected with siRNA-control (si-ctrl) or siRNA-TRIM34 (right panel) for 24 h. Then, cells were infected with IAV (MOI = 1) for 24 h or left uninfected. Co-IP and immunoblot analysis were performed with the indicated antibodies. All experiments were repeated at least three times. Bar graphs present means ± SD (∗∗p < 0.01, n.s., not significant). Co-IP, co-immunoprecipitation; IAV, influenza A virus; qPCR, quantitative RT-PCR; TRIM, tripartite motif; ZBP1, Z-DNA-binding protein 1.