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. 2022 Jan 24;298(3):101630. doi: 10.1016/j.jbc.2022.101630

Figure 4.

Figure 4

CDCP1 promotes HGF-induced membrane ruffling in T47D cells.A, T47D cells expressing MET with or without CDCP1 were stimulated with HGF and observed under time-lapse microscopy. Phase contrast images at the indicated time after HGF stimulation are shown. Open arrowheads indicate a ruffled membrane. The scale bar represents 20 μm. Fluorescence images of Lifeact-GFP are shown in Movie S1–S4. B, T47D cells expressing MET with or without CDCP1-myc were stimulated with HGF for 15 min. The distribution of F-actin was analyzed by confocal microscopy. The bottom and top views are shown. The scale bar represents 20 μm. Depth of x–z, 10 μm (C) T47D cells expressing MET with or without CDCP1-myc were stimulated with HGF for the indicated time. The activity of RAC1 was determined by a pull-down assay, followed by immunoblot analysis for GTP-RAC1 and Total RAC1. D, signal intensities of the above blots. The mean ratios ± SD were obtained from four independent experiments. ∗p < 0.05; unpaired two-tailed t test.