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. 2022 Jan 24;298(3):101630. doi: 10.1016/j.jbc.2022.101630

Figure 6.

Figure 6

ARHGEF7 is critical for CDCP1-dependent promotion of HGF-induced cell invasion in MDA-MB-231 cells.A, MDA-MB-231 cells were treated with the indicated siRNAs, and cell lysates were subjected to immunoblot analysis for ARHGEF7 and CDCP1 and MET. B, MDA-MB-231 cell lines used in the above experiments were subjected to a Boyden chamber invasion assay, and relative numbers of invaded cells were shown. C, MDA-MB-231 cell lines transfected with the indicated siRNAs were treated with HGF for the indicated time, and the activity of RAC1 was determined by a pull-down assay. D, quantification of RAC1 activity. E, MDA-MB-231 cells were treated with the indicated siRNA and then stimulated with HGF for 6 h. Cells were subjected to immunofluorescence staining for F-actin. Yellow arrowheads indicate lamellipodia. The scale bar represents 10 μm. F, ratio of the length of lamellipodia to that of total peripheral membrane. In (B, D, and F), the mean ratios ± SD were obtained from three independent experiments. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; unpaired two-tailed t test.