Figure 7.
Myofibrils from cardiomyocyte induced pluripotent stem cells (cm-iPSCs). Mant-ATP dissociation assay repeated at 20 °C for stem cell myofibrils. Without mavacamten the fast (white bars) and slow phases (black bars) had an average relative population of 58.0% and 42.0%, respectively. The average fast phase kobs = 0.02749 s−1 and the average slow phase kobs = 0.00162 s−1. When mavacamten was added (3 μM), the populations changed to 26.6% and 73.4% respectively, while the average kobs = 0.03847 s−1 and 0.00174 s−1 for the fast and slow phases, respectively. With mant-dATP added in place of mant-ATP, the population of fast and slow phase changed to 94.0% and 6.0% respectively with the average kobs = 0.07204 s−1 and 0.00142 s−1 for the fast and slow phases, respectively. With both mant-dATP and mavacamten present, the data were similar to the control values. All amplitudes and rate constants are listed in Table S1.