Figure 7.

Identification of additional autoantibody reactive region in PLA2R.A, the 1D4-tagged PLA2R extracellular domain fragments were resolved by 4 to 20% SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the PLA2R-Ab₁ dominant serum (from patient 1) at 1:5000 dilutions in TBSTM. B, the membrane was stripped and reprobed with the 1D4-Ab. C, in separate experiments, the 1D4-tagged CysR-CTLD3 fragment was resolved under nonreducing and reduced conditions and probed by the patient serum or 1D4-Ab. Each experiment was performed 3 to 5 times. 1D4-Ab, mouse anti-1D4 monoclonal antibody; PLA2R, phospholipase A2 receptor.