Table 2.

Primer sequences of the target and reference genes^a,b.

Genes symbol	Nucleotide sequence of primers (5'−3')	Product size	Accession
β-actin	F: GGCGCCCAGCACGAT	66	XM_021086047.1
	R: CCGATCCACACGGAGTACTTG
GPR41	F: TCTTCACCACCGTCTATCTCAC	398	NM_001315601.1
	R: CACAAGTCCTGCCACCCTC
GPR43	F: CTGCCTGGGATCGTCTGTG	249	XM_021093196.1
	R: CATACCCTCGGCCTTCTGG
Caspase3	F: TCTAAGCCATGGTGAAGAAGGAAAAA	112	NM_214131.1
	R: GGGTTTGCCAGTTAGAGTTCTACAG
IL-10	F: CACGGCCTTGCTCTTGTTTT	148	NM_214041.1
	R: CCTGGAAGACGTAATGCCGA
TGF-β	F: CTGACCCGCAGAGAGGCTAT	102	NM_214015.2
	R: AGAATTGAACCCGTTAATTTCCACG
Bax	F: TGACGGCAACTTCAACTGGG	143	XM_013998624.2
	R: GCAGCCGATCTCGAAGGAAGT
Bcl2	F: GAGGATTGTGGCCTTCTTTGAGT	155	XM_021099593.1
	R: CATCCCAGCCTCCGTTATCC
Foxp3	F: TCAGACCAACAGGGAGCCAA	88	XM_021079539.1
	R: TCAAGGAGGAAGAGGAGGCG
HDAC9	F: CAACAGAACGGATGGGGTGG	128	XM_021102511.1
	R: GGTCTAAAGGCGAGATGGGC
NLRP3	F: GGAGGAGGAGGAAGAGGAGATA	147	NM_001256770.2
	R: AGGACTGAGAAGATGCCACTAC
IL18	F: AGTAACCATCTCTGTGCAGTGT	155	NM_213997.1
	R: TCTTATCATCATGTCCAGGAAC
Caspase1	F: GAAGGAGAAGAGGAGGCTGTT	268	NM_214162.1
	R: AGATTGTGAACCTGTGGAGAGT
ASC	F: ACAACAAACCAGCACTGCAC	126	XM_003124468.5
	R: CTGCCTGGTACTGCTCTTCC
IL1β	F: TCTGCCCTGTACCCCAACTG	64	XM_021085847.1
	R: CCAGGAAGACGGGCTTTTG

^aF, forward primer sequence (5′ → 3′); R, reverse primer sequence (5′ → 3′).

^bGene: GPR41, G protein-coupled receptor 41; GPR43, G protein-coupled receptor 43; Caspase3, Cysteine-requiring aspartate protease 3; IL 10, Interleukin 10; TGF-β, transforming growth factor-β; Bax, BCL2-Associated X; Bcl2, B cell lymphoma 2; Foxp3, Forkhead box P3; HDAC9, Histone deacetylase 9; NLRP3, Nod-like receptor, pyrin domain 3 containing; IL 18, Interleukin 18; Caspase1, Cysteine-requiring aspartate protease 1; ASC, Apoptosis-associated speck-like protein containing CARD; IL-1β, Interleukin 1β.