Figure 4.

SMP2 regulates A3'SS in RVE8 and inhibits the expression of RVE8-activated genes. (A) Simplified diagram of the diurnal entrainment of seedlings. Plants were entrained in the diurnal cycles (12 h light/12 h dark) for 6 days and then transferred to continuous white light (145 μmol·m⁻²·s⁻¹). Seedlings were harvested at the indicated time points. (B) The schematic graph of splice variants of RVE8 in intron 7. Red arrows indicated primers used to amplify specific RVE8 transcript isoforms. Exons were depicted as black rectangles; introns were depicted as horizontal lines; alternative splicing regions were depicted as gray rectangles. (C–E) The relative abundance of three RVE8 isoforms in Col-0 and smp2-3. Total RNA was extracted from Col-0 and smp2-3 seedlings treated as described in (A). PP2A was used as the internal control. (F–I) The relative expressions of PRR5, TOC1, ELF4, and GI in Col-0 and smp2-3. Error bars represent SD, n = 3. All of these experiments were performed three times with similar results.