Figure 5.

a) Schematic illustration of the timeline for tumor model implantation with different tumor sizes and SPNCs‐mediated tumor oxygenation in the regular and large tumor models. b) Immunofluorescence staining and c,d) MFI of HIF‐1α in the regular tumor model (100 mm³) and large tumor model (360 mm³). Blue fluorescence indicated the cell nucleus, and the red fluorescence indicated the signal from anti‐HIF‐1α antibody. Scale bar = 100 µm. e) Immunofluorescence staining and f,g) MFI of pimonidazole in the regular tumor model (100 mm³) and the large tumor model (360 mm³). Blue fluorescence indicated the cell nucleus, and the green fluorescence indicated the signal from anti‐pimonidazole. Scale bar = 100 µm. Error bars represent the standard deviations of three separate measurements (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001.