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. 2022 Jan 2;9(6):2104786. doi: 10.1002/advs.202104786

Figure 4.

Figure 4

Regulatory effects of CTCF and rs2535629 on GLT8D1, NEK4, and SFMBT1. a) CTCF knockdown in U251 cells affected GLT8D1, SFMBT1, and NEK4 expression significantly. b) CTCF knockdown in SH‐SY5Y cells up‐regulated expression of GLT8D1, SFMBT1, and NEK4 significantly. c–f) rs2535629 knockout up‐regulated expression of GLT8D1, SFMBT1, and NEK4 in SH‐SY5Y cells. (c) Schematic diagram of rs2535629 knockout. The fragment length of wildtype cells is 527 bp. However, the fragment length of the genomic sequence containing rs2535629 in knocked‐out cells is 135 bp (as a 392 bp sequence containing rs2535629 was deleted by CRISPR‐Cas9). n = 3 for each group. Two‐tailed Student's t‐test was used for detection of statistical significance, *P < 0.05, **P <0.01, ***P < 0.001.