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. 2022 Feb 10;11:662723. doi: 10.3389/fonc.2021.662723

Figure 3.

Figure 3

TREM-2 is expressed in monocytic MDSC (Mo-MDSC) and tumor-associated macrophages (TAM), and a possible ligand is expressed in TAM. (A) sTREM-2 levels in the plasma of naïve, 4TI-bearing mice or in the wash of 4T1 excised tumors. (B, C) Trem2 expression by qPCR in B6 macrophages, 4T1 cells, splenic MDSC, or TAM as indicated. (B) Relative Trem2 mRNA expression in B6 macrophages compared to 4TI cells demonstrating a neglectable Trem1 expression in tumor cells. (C, D) Relative Trem2 and Trem1 mRNA expression in sorted splenic PMN-MDSC, Mo-MDSC, or TAM sorted from 4T1 tumors removed from mice (see Supplementary Figures S2 – S4 for the gating strategy). Data are normalized to a Trem1- and Trem2-expressing macrophage cell line (B6 Line). (E) Single-cell suspensions from 4T1 tumors from tumor-bearing mice were stained with MDSC markers and human (hu) IgG-Fc (gray shaded), TREM-1/hu IgG-Fc (dashed line), or TREM-2/hu IgG-Fc (solid line, no shading), followed by PE-labeled anti-human IgG-Fc. The TREM/hu IgG-Fc expression is shown on gated Gr-1-CD11b+ TAM.