Table 1.

PICO/PECO for study objectives

PICO/PECO for objective 1
Population	Human studies (adult and paediatric)
Intervention/Exposure	Omega-3s (total omega-3 or various types; supplemental and/or dietary intake)
Comparison	Genetic variation
Outcomes	HDL-cholesterol, LDL-cholesterol, LDL particle size, total cholesterol, apolipoproteins and/or TG
PICO/PECO for objective 2*
Population	Human studies (adult and paediatric)
Intervention/Exposure	Omega-3s (total omega-3 or various types; supplemental and/or dietary intake)
Comparison	Genetic variation in the same genetic location (gene(s) and SNP(s))
Outcomes	The same outcome of interest among studies with the same genetic comparators: HDL-cholesterol, LDL-cholesterol, LDL particle size, total cholesterol, apolipoproteins and/or TG

*Nutrigenetic associations/interactions were included in objective 2, in the evidence grading process, irrespective of the findings, provided that they had been reported in at least two independent studies on the same gene(s) and SNP(s), and the same plasma outcome.

HDL, high-density lipoprotein; LDL, low-density lipoprotein; SNPs, single nucleotide polymorphisms; TG, triglycerides.