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. Author manuscript; available in PMC: 2022 Feb 24.
Published in final edited form as: Neuron. 2020 Apr 9;106(6):952–962.e5. doi: 10.1016/j.neuron.2020.03.012

Figure 1. Biochemistry, functional reconstitution and overall architecture of the Torpedo nicotinic acetylcholine receptor in complex with α-bungarotoxin.

Figure 1.

(A) SDS-PAGE of affinity chromatography purified receptor. (B) Liposome patch clamp recording from receptor purified as for EM, in the same lipids used in structural analysis and in the presence of carbachol. (C) Three sections from one continuous recording of an excised patch after liposome reconstitution. Upper trace is apo receptor. Middle trace is after adding carbachol dropwise to the bath solution. Lower trace is after adding α-bungarotoxin dropwise to the bath solution. (D, E) Top and side views of cryo-EM density map. (F, G) Top and side views of atomic model.