KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological Samples
Electroplaque tissue from Torpedo californica	EastCoast Bio	Cat#DZ800
Chemicals, Peptides
2-[(4-aminobutanoyl)amino]-N,N,N-trimethylethanaminium (ATM)	This paper	N/A
N-ethylmaleimide (NEM)	Sigma	Cat#E3876
cOmplete Mini	Sigma	Cat#11836153001
Ethylenediaminetetraacetic acid (EDTA)	Sigma	Cat#E9884
Sucrose	Sigma	Cat#S0389
Triton X-100	Acros	Cat#327371000
n-Dodecyl-β-D-Maltopyranoside (DDM)	Anatrace	Cat#D310
2-Mercaptoethanol (βME)	Sigma	Cat#M6250
Soy extract polar lipids	Avanti	Cat#541602
Fos-choline-8, fluorinated	Anatrace	Cat#F300F
α-Bungarotoxin	Tocris	Cat#2133
Critical Commercial Assays
NHS-activated Sepharose 4 Fast Flow	GE Healthcare	Cat#17–0906-01
Superose 6 Increase 10/300 GL	GE Healthcare	Cat#29–0915-96
Deposited Data
Coordinates of Torpedo nicotinic receptor in complex with α-bungarotoxin	This paper	PDB: 6UWZ
Cryo-EM map of Torpedo nicotinic receptor in complex with α-bungarotoxin	This paper	EMDB: EMD-20928
Software and Algorithms
Serial EM	Mastronarde, 2005	http://bio3d.colorado.edu/SerialEM/
MotionCor2	Zheng et al., 2017	https://emcore.ucsf.edu/cryoem-software
GCTF	Zhang, 2016	https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/
Relion 3.0	Zivanov et al., 2018	https://www3.mrc-lmb.cam.ac.uk/relion/
Swissmodel	Schwede et al., 2003	https://www.swissmodel.expasy.org/
UCSF Chimera	Petterson et al., 2004	https://www.cgl.ucsf.edu/chimera/
Coot	Emsley et al., 2010	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Phenix	Afonine et al., 2018	https://www.phenix-online.org/
Pymol	Schrodinger, LLC	http://www.pymol.org/
HOLE	Smart et al., 1996	http://www.holeprogram.org/
pClamp 10	Molecular Devices	https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite
Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/
Others
Quantifoil R1.2/1.3 Au 300 mesh	Electron Microscopy Sciences	Cat#Q3100AR1.3