Table 3.
Median and inter-quartile ranges (IQR) of percent marker expression on IR1 and IR1neg Tregs after 72h in vitro IL-2 exposure of PBMC.
| HD (n=8) | IR1 cells | IR1neg Tregs | IR1/IR1neg cell ratio | |||||
|---|---|---|---|---|---|---|---|---|
| 1. Quartile | Median | 3. Quartile | 1. Quartile | Median | 3.Quartile | Ratio | p<0.01**, p<0.05* | |
| CD62L | 65.1 | 76.3 | 83.8 | 3.9 | 7.5 | 12.7 | 10.17 | 0.0078 ** |
| LAP | 89.4 | 93.0 | 96.8 | 9.8 | 11.9 | 24.0 | 7.82 | 0.0078 ** |
| IL-10 | 79.4 | 90.3 | 96.3 | 12 | 17.3 | 22.6 | 5.22 | 0.0078 ** |
| CTLA-4 | 55.3 | 65.9 | 73.7 | 7.9 | 13.7 | 31.6 | 4.81 | 0.0078 ** |
| HLA-DR | 71.9 | 87.5 | 93.4 | 22.7 | 28.6 | 32.8 | 3.06 | 0.0078 ** |
| GARP | 73 | 78.1 | 84.0 | 23.1 | 29.8 | 36.8 | 2.62 | 0.0078 ** |
| ICOS | 85.8 | 88.8 | 92.3 | 23.1 | 39.3 | 49.1 | 2.26 | 0.0312 * |
| HELIOS | 84 | 91.5 | 91.7 | 43.4 | 44.9 | 46.2 | 2.04 | 0.0078 ** |
| CD39 | 73.7 | 86.0 | 93.2 | 34.2 | 47.5 | 59.9 | 1.81 | 0.0156 * |
| CCR7 | 79.7 | 81.4 | 83.8 | 59.4 | 69.0 | 70.2 | 1.18 | 0.0312 * |
| CD45RO | 97.9 | 98.3 | 99.0 | 84.7 | 96.5 | 99.0 | 1.02 | 0.0859 ns |
Wilcoxon matched pairs, sign-rank test was used to compare median percent positive IR1 and IR1neg Tregs for each marker.
IR1/IR1neg Tregs ratio of percent cells positive for the marker.
*p < 0.05.
**p < 0.01.
ns, not significant.