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TABLE 1.

Phenotypic differences between patient isolates and type and reference strains of M. fortuitum complexa

Characteristic M. fortuitum ATCC 6841T M. peregrinum ATCC 14467T M. fortuitum third biovariant complex
Patient isolates (n = 4)
d-sorbitol-positive strain ATCC 49403 d-sorbitol-negative strain ATCC 49935
Growth at 42°C + +
Hydrolysis of acetamide + + +
Acid production from:
d-Mannitol + + + +
 i-myo-Inositol + + +
d-Sorbitol +
Resistance tob:
 Erythromycin (MIC, ≥8 μg/ml) + + + +
 Minocycline (MIC, ≥16 μg/ml) + + +
 Doxycycline (MIC, ≥16 μg/ml) + + +
 Vancomycin (MIC, ≥32 μg/ml) + + + +
 Kanamycin (zone size, <15 mm) +
 Tobramycin (zone size, <20 mm) + + + +
 Neomycin (zone size, <20 mm) +
a

Symbols: +, positive; −, negative. 

b

The MIC breakpoints for resistance that were used are those of NCCLS (8). Disk contents were as follows: kanamycin, 30 μg; tobramycin, 10 μg; and neomycin, 30 μg. Zone sizes for resistance are those of Grange and Stanford (4).