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Percentage of contacts formed or broken determined as the difference in average number of the contacts in the Ca2+-bound (A) or Mg2+-bound (B) holo state with respect to the apo states. Contacts were measured as a percentage of time that the alpha carbons of residues were within 7.0 Å of one another. A contact formed in the holo state is indicated by warm colors above the diagonal, while a contact broken in the holo state is indicated by cool colors below.
