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Co-culture live/dead comparison. Live/dead images of co-culture mixtures at day 35 (n = 3). Live cells fluoresce green when incubated with calcein AM and dead cells fluoresce red when incubated with propidium iodide. Images were taken with an Axiovert microscope. (A) Dead, (B) live, and (C) merged. Scale bars represent 500 μm. Confocal image (D) of day 1 (n = 3) merged live/dead stains; scale bars: 200 μm.
