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. 2022 Feb 11;7(7):5908–5920. doi: 10.1021/acsomega.1c06102

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© 2022 The Authors. Published by American Chemical Society

Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Co-culture gene expression rates. Data are shown as a mean of the logarithm of 2^–ΔΔCT values for the relative gene expression of genes of interest (n = 6). Bar chart demonstrates the statistical significance of gene expression comparing the co-culture MSC/PC ratios of 1:0, 3:1, 1:1, and 0:1 (cultured in differentiation media). These were also compared to MSC-CM monocultures. Data normalized to day 1 of the corresponding cell ratio. Error bars represent standard deviation between biological replicates, and significance was calculated with one-way ANOVA, where * = <0.05, ** = <0.01, and *** = <0.001.