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Co-culture day 35, qPCR. Data are shown as the mean of the logarithm of 2–ΔΔCT values for the relative expression of genes of interest (n = 6). Bar charts demonstrate statistical significance of gene expression comparing co-culture MSC/PC ratios of 1:0, 3:1, 1:1, and 0:1 cultured in differentiation media at day 35. Error bars represent standard deviation between biological replicates, and significance was calculated with one-way ANOVA, where * = <0.05, ** = <0.01, and *** = <0.001.
