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MSC culturing comparison—day 35, qPCR. Data are shown as the mean of the logarithm of 2–ΔΔCT values for relative expression of genes of interest (n = 6). Bar charts demonstrate statistical significance of gene expression comparing MSCs in differentiation media to MSCs in MSC-CM at day 35. Error bars represent standard deviation between the biological replicates, and significance was calculated with Student’s t test, where * = <0.05, ** = <0.01, and *** = <0.001.
