Figure 1.

Histology and immunofluorescent analysis. Observation of the gonads of XY male common carp at different developmental stages by H&E staining and immunofluorescence. (A) show the diagram illustrating the important biological events that occurred at six different testis developmental stages; (B–G) represent the gross morphology of fish samples at stages 1-6; (H–M) represent H&E staining results for the gonad at stages 1-6; (N–S) refer to the immunofluorescence results after staining with anti-VASA and anti-SCP3 antibodies at stages 1-6; Arrows, PGC; SG, spermatogonia; SC, spermatocytes; ST, spermatids; SZ, spermatozoa; Bar in (B–G), (H–J) and (H'–J'), (K–M) and (K'–M'), and (N–S) represent 2 cm, 10 µm, 40 µm, and 20 µm, respectively. The sections incubated without primary antibodies did not show any immunostaining (not shown).