Skip to main content
. 2022 Feb 8;12:820463. doi: 10.3389/fendo.2021.820463

Figure 4.

Figure 4

Comparison of the alternative splicing (AS) patterns in the testes and the brain during gonad development of C. carpio. (A) Statistics of categories of AS events and associated genes identified in the gonad and the brain across time course. A3SS, alternative 3ʹ splice site; A5SS, alternative 5ʹ splice site; RI, intron retention; SE, exon skipping. (B) The FPKM expression levels of genes from SE events in the testes and brain at different stages based on RNA-seq. (C) Heatmap showing numbers of differential AS events between consecutive time points in either the brain or the gonad. (D) The case of the SE pattern of the robo2 gene and validation using RT-PCR in the testes and brain of C. carpio. The expressions of the robo2 exons are shown with sequence coverage depth on the gene loci. (E) The case of the SE pattern of epb41l3b gene and its validation using RT-PCR in testes and brain of C. carpio. The expressions of the epb41l3b exons are shown with sequence coverage depth on the gene loci. (F) The case of the SE pattern of the epb41l3b gene in the gonad and brain of Danio rerio.