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. 2022 Feb 8;12:820463. doi: 10.3389/fendo.2021.820463

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Copyright © 2022 Zhao, Chen, Liu, Jiang, Chen, Chen, Song, Tao, Cui, Li, Zhu, Chen, Hu and Luo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Weighted gene co-expression network analysis (WGCNA) reveals discrete gene networks across testicular development. (A) WGCNA cluster dendrogram of time series samples identified genes that were assigned into co-expression modules. Based on similarities in module gene expression, hierarchical cluster tree with showing 33 modules of co-expressed genes. Each of the 48336 genes is represented by a leaf in the tree, and each of the 33 modules by a major tree branch. The lower panel shows modules in designated colors, such as ‘Blue’, ‘Pink’, ‘Turquoise’, and others. Genes within the gray-shaded boxes were not assigned to a module. (B) Cluster analysis of genes from the most significant module. Heatmap showing FPKM expression level of these genes. (C) The GO enrichment analysis of genes from the most significant module. (D) The KEGG enrichment analysis of genes from the most significant module. (E) Cytoscape representation of co-expressed genes from the ‘lightcyan’ module.